Studies on Madariaga virus (MADV), a potential emerging threat
MADV, an underexplored member of the eastern equine encephalitis virus complex, poses a health threat to humans and equines. In 2019, we isolated MADV from horses that died of neurological disease in Northeast Brazil. We are currently conducting various studies to better understand the transmission cycle, pathogenicity/virulence, and distribution of the virus, as well as assessing whether certain mosquito species are able to transmit the virus.
Region in Brazil where we isolated MADV in 2019 (Fig. published in Gil et al. Pathogens, 2021).
Exploring epidemiological aspects of Zika, dengue and chikungunya viruses within human cohorts
We have been conducting research in Northeast Brazil with human cohorts to study various epidemiological aspects of Zika, dengue, and chikungunya viruses. Our research has involved detecting ongoing outbreaks, exploring the role of sexual transmission in Zika epidemiology, and assessing humoral immunity profiles against arboviruses over time. These studies aim to help us better understand the spatiotemporal variation in arboviral disease epidemiology.
Hotspots of Zika virus and chikungunya virus infections during outbreaks in a region of Northeast Brazil in 2015-2016 (Fig. published in Magalhaes et al. Plos Negl Trop Dis 2017).
The relevance of sexual transmission of Zika virus was assessed through a retrospective cohort study conducted in households in Northeast Brazil (Magalhaes et al. J Infect Dis, 2021).
Field simulations: mosquito biology and arbovirus transmission
From a mosquito biology standpoint, various scenarios can occur in the field when arboviruses are circulating within a geographic area. For instance, female mosquitoes can ingest different arboviruses from different hosts, as well as antibodies and even medicine taken by humans. In the laboratory, we simulate these scenarios to assess virus transmission outcomes. This data can contribute to our understanding of disease epidemiology and may be used for modeling studies on arboviral disease transmission.
The biology of mosquito vectors must be considered in the epidemiology and transmission patterns or arboviruses. In our laboratory, we simulate various field scenarios and incorporate mosquito biology features in the experiments to evaluate arbovirus transmission outcomes.
Publications
2024
Magalhaes T, Hamer GL, de Carvalho-Leandro D, Ribeiro VML, Turell MJ. Uncertainties Surrounding Madariaga Virus, a Member of the Eastern Equine Encephalitis Virus Complex. Vector Borne Zoonotic Dis. 2024 May 8.
Castanha PMS, McEnaney PJ, Park Y, Bouwer A, Chaves EJF, Lins RD, Paciaroni NG, Dickson P, Carlson G, Cordeiro MT, Magalhaes T, Craigo J, Marques ETA, Kodadek T, Burke DS. Identification and characterization of a nonbiological small-molecular mimic of a Zika virus conformational neutralizing epitope. Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2312755121.
Magalhaes T, Barreto A, Mesquita-Silva J, Serravalle KML, Valente de Andrade M, Gomes RCL, Nazare RJ, Calado RMGA, Ribeiro GS, Kitron U. Tailoring a One Health course for an established non-One Health graduate program in Brazil. Front Trop Dis. 2024 January 5.
doi: 10.3389/fitd.2023.1317092
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Gomez G, Hufstedler H, Montenegro Morales C, Roell Y, Lozano-Parra A, Tami A, Magalhaes T, Marques ETA, Balmaseda A, Calvet G, Harris E, Brasil P, Herrera V, Villar L, Maxwell L, Jaenisch T, ReCo DIDAhsg. Pooled Cohort Profile: ReCoDID Consortium's Harmonized Acute Febrile Illness Arbovirus Meta-Cohort. JMIR Public Health Surveill. 2024;10:e54281. Epub 20240723.
doi: 10.2196/54281
2023
Magalhaes T, Portilho MM, Moreira PSS, Marinho ML, Dias WP, Gonçalves NM, Rodrigues OAS, Montes J, Reis L, Jesus DF, Silva TO, Dultra LS, Nery JS, Ribeiro GS. Validation of the use of dried blood spots in a chikungunya virus IgG serological assay. J Immunol Methods. 2023 Nov; 522: 113571.
Rosenberger KD, Phung Khanh L, Tobian F, Chanpheaktra N, Kumar V, Lum LCS, Sathar J, Pleites Sandoval E, Maron GM, Laksono IS, Mahendradhata Y, Sarker M, Rahman R, Caprara A, Souza Benevides B, Marques ETA, Magalhaes T, Brasil P, Amaral Calvet G, Tami A, Bethencourt SE, Dong Thi Hoai T, Nguyen Tan Thanh K, Tran Van N, Nguyen Tran N, Do Chau V, Yacoub S, Nguyen Van K, Guzman MG, Martinez PA, Nguyen Than Ha Q, Simmons CP, Wills BA, Geskus RB, Jaenisch T, International Research Consortium on Dengue Risk Assessment M, Surveillance I. Early diagnostic indicators of dengue versus other febrile illnesses in Asia and Latin America (IDAMS study): a multicentre, prospective, observational study. Lancet Glob Health. 2023;11(3):e361-e72.
doi: 10.1016/S2214-109X(22)00514-9
Foy BD, Magalhaes T, Some A, Gray L, Rao S, Sougue E, Jackson CL, Kittleson J, Slater HC, Bousema T, Ollo D, Coulidiaty G, Colt M, Wade M, Richards K, Fabrice Some A, Dabire RK, Parikh S. Repeat Ivermectin Mass Drug Administrations for Malaria Control II: a double-blind, cluster-randomized control trial for integrated control of malaria. Protocol for the RIMDAMAL II Trial. JMIR Research Protocols 2023; 12:1-13.
doi : 10.2196/41197
2022
Magalhaes T, Morais CNL, Azevedo EAN, Jacques IJAA, Castanha PMS, Cordeiro MT, Braga C, Jaenisch T, Marques ETA, Foy BD. Two-year decay of Zika virus neutralizing antibodies in people living in an endemic region in Brazil. Am J Trop Med Hyg 2022; 107(1):186-189.
doi: 10.4269/ajtmh.21-1279
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Cardoso CW, Souza MMS, Teles ACVS, Argibay HD, Reis OB, Pereira FM, Giovanetti M, Magalhaes T, Ribeiro GS. COVID-19 outbreaks among crew members in non-cruise vessels anchoring in Salvador, Brazil, 2021. Mem Inst Oswaldo Cruz 2022; 117:1-8.
2021
Gil L*, Magalhaes T*, Santos B, et al. Active Circulation of Madariaga Virus, a Member of the Eastern Equine Encephalitis Virus Complex, in Northeast Brazil. Pathogens 2021; 10. *Equal contribution
doi: 10.3390/pathogens10080983
Magalhaes T, Morais CNL, Jacques I, et al. Follow-Up Household Serosurvey in Northeast Brazil for Zika Virus: Sexual Contacts of Index Patients Have the Highest Risk for Seropositivity. J Infect Dis 2021; 223:673-85.
doi: 10.1093/infdis/jiaa563
Loeffler FF, Viana IFT, Fischer N, et al. Identification of a Zika NS2B epitope as a biomarker for severe clinical phenotypes. RSC Med Chem 2021; 12:1525-39.
doi: 10.1039/d1md00124h
Morales I, Rosenberger KD, Magalhaes T, et al. Diagnostic performance of anti-Zika virus IgM, IgAM and IgG ELISAs during co-circulation of Zika, dengue, and chikungunya viruses in Brazil and Venezuela. PLoS Negl Trop Dis 2021; 15:e0009336.
2020
Magalhaes T, Chalegre KDM, Braga C, Foy BD. The Endless Challenges of Arboviral Diseases in Brazil. Trop Med Infect Dis 2020; 5.
doi: 10.3390/tropicalmed5020075
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Blitvich BJ, Magalhaes T, Laredo-Tiscareno SV, Foy BD. Sexual Transmission of Arboviruses: A Systematic Review. Viruses 2020; 12.
doi: 10.3390/v12090933
Armstrong PM, Ehrlich HY, Magalhaes T, et al. Successive blood meals enhance virus dissemination within mosquitoes and increase transmission potential. Nat Microbiol 2020; 5:239-47.
doi: 10.1038/s41564-019-0619-y
2019
Magalhaes T, Bergren NA, Bennett SL, et al. Induction of RNA interference to block Zika virus replication and transmission in the mosquito Aedes aegypti. Insect Biochem Mol Biol 2019; 111:103169.
doi: 10.1016/j.ibmb.2019.05.004
Foy BD, Alout H, Seaman JA, et al. Efficacy and risk of harms of repeat ivermectin mass drug administrations for control of malaria (RIMDAMAL): a cluster-randomised trial. Lancet 2019:10.
2018
Magalhaes T, Robison A, Young MC, et al. Sequential Infection of Aedes aegypti Mosquitoes with Chikungunya Virus and Zika Virus Enhances Early Zika Virus Transmission. Insects 2018; 9.
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Magalhaes T, Foy BD, Marques ETA, Ebel GD, Weger-Lucarelli J. Mosquito-borne and sexual transmission of Zika virus: Recent developments and future directions. Virus Res 2018; 254:1-9.
2017
Magalhaes T, Braga C, Cordeiro MT, et al. Zika virus displacement by a chikungunya outbreak in Recife, Brazil. PLoS Negl Trop Dis 2017; 11:e0006055.
doi: 10.1371/journal.pntd.0006055
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Faria NR, Quick J, Claro IM, et al. Establishment and cryptic transmission of Zika virus in Brazil and the Americas. Nature 2017; 546:406-10.
doi: 10.1038/nature22401
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Chotiwan N, Brewster CD, Magalhaes T, et al. Rapid and specific detection of Asian- and African-lineage Zika viruses. Sci Transl Med 2017; 9.
doi: 10.1126/scitranslmed.aag0538
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Bosch I, de Puig H, Hiley M, et al. Rapid antigen tests for dengue virus serotypes and Zika virus in patient serum. Sci Transl Med 2017; 9.
doi: 10.1126/scitranslmed.aan1589
Kassar TC, Magalhaes T, Junior SJ, et al. Construction and characterization of a recombinant yellow fever virus stably expressing Gaussia luciferase. An Acad Bras Cienc 2017; 89:2119-30.
doi: 10.1590/0001-3765201720160196
Freire M, Pol-Fachin L, Coelho DF, et al. Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses. ACS Omega 2017; 2:3913-20.
doi: 10.1021/acsomega.7b00608